4C1P

Geobacillus thermoglucosidasius GH family 52 xylosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-06
Detector	RIGAKU SATURN 944
Spacegroup name	C 2 2 21
Unit cell lengths	73.369, 105.335, 194.834
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.311 - 2.634
R-factor	0.1852
Rwork	0.182
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NATIVE STRUCTURE
RMSD bond length	0.003
RMSD bond angle	0.805
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	REFMAC
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.640	2.640
Rmerge	0.140	0.480
Number of reflections	22164
<I/σ(I)>	7.62	1.73
Completeness [%]	97.3	78
Redundancy	5	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7		10MG/ML BETA-XYLOSIDASE IN 50MM TRIS-HCL PH8, 150MM NACL, 100MM XYLOSE WAS MIXED EQUIVOLUME WITH 0.1M MES PH6, 0.4M AMMONIUM SULPHATE, 25% 3350. CRYSTALS WERE GROWN BY THE HANGING DROP VAPOUR DIFFUSION METHOD., pH 7