4C1N

Corrinoid protein reactivation complex with activator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-15
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 1
Unit cell lengths	67.134, 128.175, 163.366
Unit cell angles	77.61, 82.25, 88.76

Refinement procedure

Resolution	30.631 - 2.530
R-factor	0.1934
Rwork	0.191
R-free	0.24290
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.003
RMSD bond angle	0.740
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.600
High resolution limit [Å]	2.530	2.530
Rmerge	0.090	0.540
Number of reflections	168978
<I/σ(I)>	10	2.6
Completeness [%]	96.0	85
Redundancy	2.74	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1