4C1B
Esterase domain of the ZfL2-1 ORF1 protein from the zebrafish ZfL2-1 retrotransposon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-09-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0, 1.0397 |
| Spacegroup name | I 41 |
| Unit cell lengths | 111.260, 111.260, 115.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.709 - 2.501 |
| R-factor | 0.1694 |
| Rwork | 0.167 |
| R-free | 0.20990 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.182 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 24355 | |
| <I/σ(I)> | 19.1 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 100 MM NA-HEPES PH=7.0, 0.5% JEFFAMINE, 1.1M NA-MALONATE. |
