4C18
The structure of the Tsi2 dimer with a disulfide bond
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2011-07-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 27.970, 36.300, 37.860 |
| Unit cell angles | 107.37, 95.23, 92.11 |
Refinement procedure
| Resolution | 34.590 - 1.490 |
| R-factor | 0.22445 |
| Rwork | 0.223 |
| R-free | 0.25886 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vpv |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.812 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.930 | 1.570 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.110 | 0.240 |
| Number of reflections | 21050 | |
| <I/σ(I)> | 7.3 | 3.7 |
| Completeness [%] | 92.0 | 88.6 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M SODIUM CITRATE PH 5.6, 35% T-BUTANOL |
