4C0T
Candida albicans PKh Kinase Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 100.210, 100.210, 60.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.859 - 3.160 |
| R-factor | 0.2305 |
| Rwork | 0.228 |
| R-free | 0.27990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hrc |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.593 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.900 | 3.260 |
| High resolution limit [Å] | 3.160 | 3.160 |
| Rmerge | 0.170 | 0.800 |
| Number of reflections | 5590 | |
| <I/σ(I)> | 14.54 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 100 MM AMMONIUM SULFATE, 30 % (W/V) PEG 4000, AND 100 MM SODIUM CITRATE/ CITRIC ACID (PH 5.6) |
