4C0S
Mammalian translation elongation factor eEF1A2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 135.370, 135.370, 304.620 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.703 |
| R-factor | 0.205 |
| Rwork | 0.202 |
| R-free | 0.25560 |
| Structure solution method | MIR |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.512 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.6.0114) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.080 | 0.860 |
| Number of reflections | 45528 | |
| <I/σ(I)> | 18.52 | 2.76 |
| Completeness [%] | 98.4 | 95.6 |
| Redundancy | 7.5 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 50 MM MES PH 5.6, 2.4 M AMMONIUM SULFATE, 10 MM MAGNESIUM ACETATE |
