4C0O
Transportin 3 in complex with phosphorylated ASF/SF2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-27 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 80.552, 91.062, 98.122 |
| Unit cell angles | 106.98, 100.30, 102.18 |
Refinement procedure
| Resolution | 38.283 - 2.557 |
| R-factor | 0.2236 |
| Rwork | 0.221 |
| R-free | 0.26910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3BEG AND 4C0P |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.924 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.300 | 2.710 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.060 | 0.710 |
| Number of reflections | 80114 | |
| <I/σ(I)> | 10.3 | 1.6 |
| Completeness [%] | 99.3 | 99.2 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 16% (W/V) PEG3350, 0.1 M KSCN, 0.1 M BIS-TRIS PROPANE, PH6.0 |
