4C0O
Transportin 3 in complex with phosphorylated ASF/SF2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-27 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 |
Unit cell lengths | 80.552, 91.062, 98.122 |
Unit cell angles | 106.98, 100.30, 102.18 |
Refinement procedure
Resolution | 38.283 - 2.557 |
R-factor | 0.2236 |
Rwork | 0.221 |
R-free | 0.26910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 3BEG AND 4C0P |
RMSD bond length | 0.005 |
RMSD bond angle | 0.924 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.300 | 2.710 |
High resolution limit [Å] | 2.570 | 2.570 |
Rmerge | 0.060 | 0.710 |
Number of reflections | 80114 | |
<I/σ(I)> | 10.3 | 1.6 |
Completeness [%] | 99.3 | 99.2 |
Redundancy | 3.4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 16% (W/V) PEG3350, 0.1 M KSCN, 0.1 M BIS-TRIS PROPANE, PH6.0 |