4C0N
Crystal structure of non symbiotic plant hemoglobin AHb3 (GLB3) from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-01-31 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 123.373, 123.373, 123.373 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.420 - 1.770 |
R-factor | 0.18675 |
Rwork | 0.186 |
R-free | 0.20530 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.014 |
RMSD bond angle | 1.510 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.300 | 1.810 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.050 | 0.770 |
Number of reflections | 31454 | |
<I/σ(I)> | 16.4 | 1.9 |
Completeness [%] | 99.0 | 99.9 |
Redundancy | 5.4 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.2 | 1.6 M AMMONIUM SULFATE, 0.1 M TRIS PH 8.2 |