4C0M
Crystal Structure of the N terminal domain of wild type TRIF (TIR- domain-containing adapter-inducing interferon-beta)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-15 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.000, 49.400, 70.910 |
| Unit cell angles | 88.56, 77.20, 72.11 |
Refinement procedure
| Resolution | 39.700 - 2.800 |
| R-factor | 0.2537 |
| Rwork | 0.252 |
| R-free | 0.29120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bsx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.970 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.510 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.200 | 0.400 |
| Number of reflections | 13337 | |
| <I/σ(I)> | 3.9 | 2 |
| Completeness [%] | 89.6 | 90.4 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M BIS TRIS PH 6.6, 150 MM NACL AND 21% PEG 3350 |
