4C0K
Crystal structure of Drosophila Miro EF hand and cGTPase domains bound to one calcium ion (Ca-MiroS)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-29 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 82.240, 82.240, 156.441 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.075 - 2.801 |
R-factor | 0.2107 |
Rwork | 0.207 |
R-free | 0.24220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | INITIAL MODEL FROM PRIOR DATASET |
RMSD bond length | 0.003 |
RMSD bond angle | 0.475 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.100 | 2.970 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.140 | 0.700 |
Number of reflections | 15693 | |
<I/σ(I)> | 11.1 | 1.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 15.1 | 15.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.4 | 5.0MG/ML MIROS, 1.7 M LISO4, 0.1 M BIS-TRIS PH 7.4, 5 MM EGTA; CRYSTAL SOAKED IN 10MM CACL2 FOR 72H PRIOR TO DATA COLLECTION. |