4C0H

Extended interface between Pcf11p and Clp1p and structural basis for ATP loss in Gly135Arg point mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	89.395, 95.609, 182.063
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.800 - 2.700
R-factor	0.1922
Rwork	0.191
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.240
Data reduction software	XDS
Data scaling software	SCALEPACK
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.860
High resolution limit [Å]	2.700	2.700
Rmerge	0.090	0.635
Number of reflections	43523
<I/σ(I)>	21.25	3.48
Completeness [%]	99.5	97.3
Redundancy	9.46	9.07

Crystallization Conditions

crystal ID	method	pH	temperature	details
1