4C0F
Structure of the NOT-box domain of human CNOT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00001,0.97945 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 64.830, 64.830, 412.290 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.304 - 2.400 |
| R-factor | 0.2193 |
| Rwork | 0.217 |
| R-free | 0.26280 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.641 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 21312 | |
| <I/σ(I)> | 13.2 | 2.4 |
| Completeness [%] | 99.4 | 99.1 |
| Redundancy | 6.7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 100MM TRI-SODIUM CITRATE PH=5.6, 10% PEG4000, 10% ISOPROPANOL |
