4BZH
Crystal structure of galactose mutarotase GalM from Bacillus subtilis in complex with maltose and trehalose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-01-29 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 82.194, 83.291, 124.754 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.430 - 2.600 |
| R-factor | 0.21677 |
| Rwork | 0.214 |
| R-free | 0.26904 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mwx |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.381 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.430 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.280 | 0.500 |
| Number of reflections | 36065 | |
| <I/σ(I)> | 4.4 | 2.3 |
| Completeness [%] | 99.0 | 93.2 |
| Redundancy | 6.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
