4BZF
Crystal structure of galactose mutarotase GalM from Bacillus subtilis with trehalose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 82.190, 83.322, 124.446 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.160 - 1.900 |
| R-factor | 0.19658 |
| Rwork | 0.194 |
| R-free | 0.23773 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mwx |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.473 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.160 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.120 | 0.500 |
| Number of reflections | 67897 | |
| <I/σ(I)> | 8.4 | 2.1 |
| Completeness [%] | 99.8 | 98.5 |
| Redundancy | 6.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
