4BZ4
CorA is a surface-associated copper-binding protein important in Methylomicrobium album BG8 copper acquisition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-10 |
Detector | ADSC CCD |
Wavelength(s) | 0.9794, 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.840, 113.170, 81.490 |
Unit cell angles | 90.00, 104.51, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.600 |
R-factor | 0.15906 |
Rwork | 0.158 |
R-free | 0.18809 |
Structure solution method | SIRAS |
Starting model (for MR) | NONE |
RMSD bond length | 0.021 |
RMSD bond angle | 2.010 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SOLVE |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.640 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.050 | 0.430 |
Number of reflections | 168264 | |
<I/σ(I)> | 15.3 | 2.8 |
Completeness [%] | 98.6 | 97 |
Redundancy | 2.8 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 11.5-14% PEG 8K, 0.1 BISTRIS PH 7 |