4BZ0
Structural characterization using Sulfur-SAD of the cytoplasmic domain of Burkholderia pseudomallei PilO2Bp, an actin-like protein component of a Type IVb R64-derivative pilus machinery.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-12 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 52.740, 52.740, 127.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.040 - 1.760 |
| R-factor | 0.19409 |
| Rwork | 0.191 |
| R-free | 0.25043 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4byz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.373 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.330 | 1.860 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.060 | 0.220 |
| Number of reflections | 16362 | |
| <I/σ(I)> | 16.6 | 5 |
| Completeness [%] | 88.0 | 99.1 |
| Redundancy | 10.3 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.9 M SODIUM POTASSIUM PHOSPHATE PH 7.0 |
