4BYF
Crystal structure of human Myosin 1c in complex with calmodulin in the pre-power stroke state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-11 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.580, 158.450, 114.340 |
| Unit cell angles | 90.00, 91.56, 90.00 |
Refinement procedure
| Resolution | 47.940 - 2.740 |
| Rwork | 0.183 |
| R-free | 0.23670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lkx |
| RMSD bond length | 0.834 |
| RMSD bond angle | 0.005 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.940 | 2.840 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.070 | 0.850 |
| Number of reflections | 51985 | |
| <I/σ(I)> | 12.4 | 2.15 |
| Completeness [%] | 94.0 | 89 |
| Redundancy | 2.4 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 18% PEG3350, 0.2 M SODIUM MALONATE PH 7.0 |
