4BY5
Crystal structure of Drosophila Frq2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-05 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.984, 131.192, 56.710 |
| Unit cell angles | 90.00, 91.07, 90.00 |
Refinement procedure
| Resolution | 42.930 - 2.220 |
| R-factor | 0.21366 |
| Rwork | 0.211 |
| R-free | 0.27052 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g8i |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.792 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.970 | 2.340 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.080 | 0.470 |
| Number of reflections | 40192 | |
| <I/σ(I)> | 12.2 | 2.6 |
| Completeness [%] | 99.4 | 96.5 |
| Redundancy | 3.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 21% PEG 4000, 0.1 M CACL2, 0.1 M HEPES PH 7.5 |
