4BY2
SAS-4 (dCPAP) TCP domain in complex with a Proline Rich Motif of Ana2 (dSTIL) of Drosophila Melanogaster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-23 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.640, 69.910, 69.980 |
| Unit cell angles | 86.96, 88.64, 67.69 |
Refinement procedure
| Resolution | 64.600 - 2.570 |
| R-factor | 0.246 |
| Rwork | 0.245 |
| R-free | 0.26310 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | autoSHARP |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.600 | 8.900 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.090 | 0.510 |
| Number of reflections | 31911 | |
| <I/σ(I)> | 7.6 | 1.7 |
| Completeness [%] | 97.6 | 97.2 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 100 MM MES/IMIDAZOLE MIX PH 6.5, 30 MM MGCL2, 30 MM CACL2, 20 % ETHYLENE GLYCOL, 10 % PEG LIQUOR, IN A 0.2UL SITTING DROP. |
