4BXW
Crystal Structure of the Prothrombinase Complex from the Venom of Pseudonaja Textilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-21 |
Detector | ADSC CCD |
Spacegroup name | I 21 3 |
Unit cell lengths | 179.310, 179.310, 179.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.200 - 2.710 |
R-factor | 0.15917 |
Rwork | 0.155 |
R-free | 0.23308 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | MODEL BASED ON PDB ENTRY 1HCG |
RMSD bond length | 0.003 |
RMSD bond angle | 0.669 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.200 | 2.850 |
High resolution limit [Å] | 2.710 | 2.710 |
Rmerge | 0.120 | 0.690 |
Number of reflections | 26001 | |
<I/σ(I)> | 10.3 | 2.4 |
Completeness [%] | 1.0 | 99.3 |
Redundancy | 5.5 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 45MM MES PH 5.5 AND 5.6% PVP K12 |