4BXT
Crystal structure of the human metapneumovirus phosphoprotein tetramerization domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-22 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.910, 48.520, 64.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.420 - 3.130 |
| R-factor | 0.236 |
| Rwork | 0.235 |
| R-free | 0.25230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SAXS-FILTERED ROSETTA FOLD-AND-DOCK AB INITIO MODEL |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.290 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.910 | 3.210 |
| High resolution limit [Å] | 3.130 | 3.130 |
| Rmerge | 0.350 | 1.350 |
| Number of reflections | 3602 | |
| <I/σ(I)> | 6.4 | 1.4 |
| Completeness [%] | 90.3 | 57.1 |
| Redundancy | 10.5 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 25 % PEG 3350, 100 MM HEPES PH 7.5 |
