4BXR
Structure of the wild-type TCP10 domain of Danio rerio CPAP in complex with a peptide of Danio rerio STIL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-13 |
Detector | DECTRIS PIXEL |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.250, 67.470, 61.650 |
Unit cell angles | 90.00, 113.92, 90.00 |
Refinement procedure
Resolution | 51.130 - 2.200 |
R-factor | 0.23642 |
Rwork | 0.234 |
R-free | 0.27668 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4BXP (DISTORTED) |
RMSD bond length | 0.015 |
RMSD bond angle | 1.619 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.400 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.100 | 1.010 |
Number of reflections | 23051 | |
<I/σ(I)> | 7.6 | 1.4 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 4.8 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 100 MM TRIS PH 8.5, 200 MM CA ACETATE, 17% PEG-2000 MME |