4BXR
Structure of the wild-type TCP10 domain of Danio rerio CPAP in complex with a peptide of Danio rerio STIL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-13 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.250, 67.470, 61.650 |
| Unit cell angles | 90.00, 113.92, 90.00 |
Refinement procedure
| Resolution | 51.130 - 2.200 |
| R-factor | 0.23642 |
| Rwork | 0.234 |
| R-free | 0.27668 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4BXP (DISTORTED) |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.619 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.400 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.100 | 1.010 |
| Number of reflections | 23051 | |
| <I/σ(I)> | 7.6 | 1.4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 100 MM TRIS PH 8.5, 200 MM CA ACETATE, 17% PEG-2000 MME |
