4BXP
Structure of the wild-type TCP10 domain of Danio rerio CPAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-09 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.340, 36.440, 56.440 |
| Unit cell angles | 90.00, 117.31, 90.00 |
Refinement procedure
| Resolution | 28.680 - 1.700 |
| R-factor | 0.20151 |
| Rwork | 0.199 |
| R-free | 0.24373 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.478 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CRANK |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.070 | 0.930 |
| Number of reflections | 21047 | |
| <I/σ(I)> | 14.6 | 2 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 80 MM TRIS PH 8.5, 160 MM MGCL2, 20% PEG4K, 18 % GLYCEROL, 1MM DTT |
