4BXP
Structure of the wild-type TCP10 domain of Danio rerio CPAP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-09 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.340, 36.440, 56.440 |
Unit cell angles | 90.00, 117.31, 90.00 |
Refinement procedure
Resolution | 28.680 - 1.700 |
R-factor | 0.20151 |
Rwork | 0.199 |
R-free | 0.24373 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.011 |
RMSD bond angle | 1.478 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CRANK |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.500 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.070 | 0.930 |
Number of reflections | 21047 | |
<I/σ(I)> | 14.6 | 2 |
Completeness [%] | 99.7 | 100 |
Redundancy | 7.2 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 80 MM TRIS PH 8.5, 160 MM MGCL2, 20% PEG4K, 18 % GLYCEROL, 1MM DTT |