4BXM
Complement regulator acquiring outer surface protein BbCRASP-4 or ErpC from Borrelia burgdorferi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 41 |
| Unit cell lengths | 62.680, 62.680, 76.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.580 - 2.150 |
| R-factor | 0.17274 |
| Rwork | 0.171 |
| R-free | 0.20821 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bod |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.955 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.320 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.060 | 0.230 |
| Number of reflections | 16062 | |
| <I/σ(I)> | 13.4 | 5 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0,1 HEPES PH7.5, 10% ISOPROPANOL, 20% PEG 6000 |
