4BXJ
CRYSTAL STRUCTURE OF AMPDH3 FROM PSEUDOMONAS AERUGINOSA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 101.805, 101.805, 164.475 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.627 - 2.350 |
R-factor | 0.2169 |
Rwork | 0.214 |
R-free | 0.25810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bj4 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.187 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.630 | 2.480 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.010 | 0.100 |
Number of reflections | 36822 | |
<I/σ(I)> | 42.6 | 5.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | pH 4.5 |