4BXH
Resolving the activation site of positive regulators in plant phosphoenolpyruvate carboxylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-12-03 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 162.918, 122.857, 131.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.000 - 2.240 |
R-factor | 0.17741 |
Rwork | 0.177 |
R-free | 0.21006 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zge |
RMSD bond length | 0.017 |
RMSD bond angle | 1.797 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.000 | 2.360 |
High resolution limit [Å] | 2.240 | 2.240 |
Rmerge | 0.050 | 0.390 |
Number of reflections | 124109 | |
<I/σ(I)> | 12.6 | 2 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 4.5 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 0.1 M TRI-SODIUM CITRATE (PH 5.6/NAOH), 15 % (W/V) PEG4000 AND 0.2 M AMMONIUM SULFATE |