4BXH
Resolving the activation site of positive regulators in plant phosphoenolpyruvate carboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 162.918, 122.857, 131.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.000 - 2.240 |
| R-factor | 0.17741 |
| Rwork | 0.177 |
| R-free | 0.21006 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zge |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.797 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 | 2.360 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.050 | 0.390 |
| Number of reflections | 124109 | |
| <I/σ(I)> | 12.6 | 2 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 0.1 M TRI-SODIUM CITRATE (PH 5.6/NAOH), 15 % (W/V) PEG4000 AND 0.2 M AMMONIUM SULFATE |
