4BX3
Crystal Structure of murine Chronophin (Pyridoxal Phosphate Phosphatase)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-23 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 167.100, 167.100, 167.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.659 - 2.193 |
| R-factor | 0.1725 |
| Rwork | 0.171 |
| R-free | 0.20670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oyc |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.640 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.660 | 2.310 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.080 | 0.860 |
| Number of reflections | 39743 | |
| <I/σ(I)> | 12.9 | 2.2 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 6.8 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1M IMIDAZOLE PH 8, 0.2M NACL, 1M SODIUM TARTRATE |
