4BWZ
Crystal structure of the sodium proton antiporter, NapA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2012-05-18 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 73.750, 82.150, 191.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.953 - 2.984 |
| R-factor | 0.2237 |
| Rwork | 0.222 |
| R-free | 0.24820 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.126 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.000 | 3.060 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 0.080 | 0.950 |
| Number of reflections | 12265 | |
| <I/σ(I)> | 18.8 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 0.001 M ZINC SULPHATE, 0.05 M HEPES PH 7.8 AND 22% PEG 600 |
