4BWY
P4 PROTEIN FROM BACTERIOPHAGE PHI8 (R32)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 197.725, 197.725, 562.576 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.390 - 3.100 |
| R-factor | 0.2961 |
| Rwork | 0.295 |
| R-free | 0.30860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4blq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.080 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.310 | 1.000 |
| Number of reflections | 76933 | |
| <I/σ(I)> | 8.6 | 1.3 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 12.7 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 100 MM SODIUM ACETATE PH 4.6 AND 2.2 M AMMONIUM SULPHATE |
