4BWP
Structure of Drosophila Melanogaster PAN3 pseudokinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-05-14 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 102.171, 102.171, 264.715 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.800 - 3.600 |
R-factor | 0.22225 |
Rwork | 0.220 |
R-free | 0.26878 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bwk |
RMSD bond length | 0.010 |
RMSD bond angle | 1.468 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.800 | 3.790 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.110 | 0.890 |
Number of reflections | 17015 | |
<I/σ(I)> | 14.5 | 2.1 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 6.4 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.8 | 220 MM NA-CITRATE PH 7.0, HEPES PH 6.8, 2% PEG 3350 |