4BWP
Structure of Drosophila Melanogaster PAN3 pseudokinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-14 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 102.171, 102.171, 264.715 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.800 - 3.600 |
| R-factor | 0.22225 |
| Rwork | 0.220 |
| R-free | 0.26878 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bwk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.468 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 3.790 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.110 | 0.890 |
| Number of reflections | 17015 | |
| <I/σ(I)> | 14.5 | 2.1 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.4 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 220 MM NA-CITRATE PH 7.0, HEPES PH 6.8, 2% PEG 3350 |
