4BWK
Structure of Neurospora crassa PAN3 pseudokinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 65 |
| Unit cell lengths | 89.521, 89.521, 228.789 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.027 - 3.300 |
| R-factor | 0.2541 |
| Rwork | 0.252 |
| R-free | 0.29370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.524 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.030 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.060 | 0.770 |
| Number of reflections | 15611 | |
| <I/σ(I)> | 19.9 | 3 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 7.9 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES PH 7.5, 7% PEG 6000, 6% ISOPROPANOL |
