4BWG
Structural basis of subtilase cytotoxin SubAB assembly
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.212, 81.712, 227.791 |
Unit cell angles | 90.00, 100.24, 90.00 |
Refinement procedure
Resolution | 38.070 - 2.600 |
R-factor | 0.2207 |
Rwork | 0.219 |
R-free | 0.24960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTIES 3DWA AND 2IY9 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.090 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.080 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.110 | 0.560 |
Number of reflections | 252165 | |
<I/σ(I)> | 11.3 | 2.3 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 3.8 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 3.1 | 11% PEG8K, 0.5M LISO4, 0.1M CITRATE PH 3.1 |