4BWG
Structural basis of subtilase cytotoxin SubAB assembly
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.212, 81.712, 227.791 |
| Unit cell angles | 90.00, 100.24, 90.00 |
Refinement procedure
| Resolution | 38.070 - 2.600 |
| R-factor | 0.2207 |
| Rwork | 0.219 |
| R-free | 0.24960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTIES 3DWA AND 2IY9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.080 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.110 | 0.560 |
| Number of reflections | 252165 | |
| <I/σ(I)> | 11.3 | 2.3 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.1 | 11% PEG8K, 0.5M LISO4, 0.1M CITRATE PH 3.1 |
