4BWE
Crystal structure of C-terminally truncated glypican-1 after controlled dehydration to 86 percent relative humidity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.230, 166.720, 139.090 |
| Unit cell angles | 90.00, 90.80, 90.00 |
Refinement procedure
| Resolution | 29.476 - 2.460 |
| R-factor | 0.2313 |
| Rwork | 0.229 |
| R-free | 0.28040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4acr |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.890 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.480 | 2.550 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.100 | 0.660 |
| Number of reflections | 75752 | |
| <I/σ(I)> | 7.6 | 2.1 |
| Completeness [%] | 97.3 | 97.3 |
| Redundancy | 2.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 13 % PEG 6000, 0.1 M TRIS-HCL PH 8 & 0.2 M CACL2 |
