4BWC
X-ray structure of a phospholiapse B like protein 1 from bovine kidneys
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 97.370, 97.370, 140.945 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.980 - 1.890 |
| R-factor | 0.19019 |
| Rwork | 0.188 |
| R-free | 0.23061 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fbx |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.181 |
| Data reduction software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.980 | 2.000 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.060 | 0.620 |
| Number of reflections | 62608 | |
| <I/σ(I)> | 18.74 | 2.91 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M TRIS-CL PH 7, 40% PEG 300, 5% PEG 1000 |
