Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-16 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 129.075, 129.075, 229.660 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.590 - 2.580 |
| R-factor | 0.17869 |
| Rwork | 0.176 |
| R-free | 0.22442 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ZGR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.141 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.500 | 2.720 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.140 | 0.800 |
| Number of reflections | 23460 | |
| <I/σ(I)> | 12.5 | 3.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.1 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.6 | THE PROTEIN WAS AT 1 MG/ML AFTER DIALYSIS WITH HEPES BUFFER AND CYANURIC ACID, THIS WAS ADDED IN A 3:1 RATIO TO THE RESERVIOR SOLUTION OF 38% V/V PEG 400 AND 100 MM HEPES PH 6.6 |
