Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-09 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 130.659, 130.659, 236.947 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.750 - 1.900 |
| R-factor | 0.17077 |
| Rwork | 0.170 |
| R-free | 0.18929 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.098 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.700 | 2.000 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.240 | 1.490 |
| Number of reflections | 61796 | |
| <I/σ(I)> | 18.9 | 4.1 |
| Completeness [%] | 99.7 | 98 |
| Redundancy | 36.7 | 18.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.7 | THE PROTEIN WAS CONCENTRATED TO 10MG/ML AND WAS SET UP IN A 50:50 DROP WITH THE RESERVOIR BEING 38% V/V PEG MME 350 AND 100 MM HEPES PH 7.7 |
