4BVK
Structure of Y190E mutant of PhaZ7 PHB depolymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-19 |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.410, 199.320, 44.310 |
| Unit cell angles | 90.00, 114.39, 90.00 |
Refinement procedure
| Resolution | 19.776 - 1.606 |
| R-factor | 0.1366 |
| Rwork | 0.135 |
| R-free | 0.17010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4brs |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.610 |
| Rmerge | 0.040 | 0.230 |
| Number of reflections | 81143 | |
| <I/σ(I)> | 19.1 | 4.7 |
| Completeness [%] | 95.5 | 85.5 |
| Redundancy | 2.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | VAPOUR DIFFUSION HANGING DROP. PROTEIN CONCENTRATION 10 MG/ML. 0.1 M NAOAC (PH 5.0), 0.2 M MGCL2, 18% W/V PEG6000. |
