4BVJ
Structure of Y105A mutant of PhaZ7 PHB depolymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-19 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.370, 200.050, 44.180 |
| Unit cell angles | 90.00, 114.28, 90.00 |
Refinement procedure
| Resolution | 20.005 - 1.599 |
| R-factor | 0.1569 |
| Rwork | 0.155 |
| R-free | 0.18870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4brs |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.082 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.070 | 0.480 |
| Number of reflections | 84896 | |
| <I/σ(I)> | 11.7 | 2 |
| Completeness [%] | 98.7 | 97.2 |
| Redundancy | 2.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | VAPOUR DIFFUSION HANGING DROP, PROTEIN CONCENTRATION 10 MG/ML, 0.1 M NAOAC (PH 5.0), 0.2 M NH4CL, 20% W/V PEG6000 |
