4BUM
Crystal structure of the Voltage Dependant Anion Channel 2 from zebrafish.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-16 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 72.470, 72.470, 177.950 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 51.286 - 2.801 |
R-factor | 0.2492 |
Rwork | 0.245 |
R-free | 0.28220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3emn |
RMSD bond length | 0.004 |
RMSD bond angle | 0.871 |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 51.290 |
High resolution limit [Å] | 2.800 |
Rmerge | 0.080 |
Number of reflections | 13686 |
<I/σ(I)> | 13 |
Completeness [%] | 98.0 |
Redundancy | 3.56 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 100 MM TRISHCL (PH=8.0), 100 MM KCL, AND PEG 2000 IN A RANGE OF 19-24%, 0.011% N-UNDECYL-BETA-D-THIOMALTOPYRANOSIDE |