4BSM
Crystal structure of the Nuclear Export Receptor CRM1 (exportin-1) lacking the C-terminal helical extension at 4.5A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-05 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 148.640, 248.330, 107.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.589 - 4.500 |
R-factor | 0.2329 |
Rwork | 0.229 |
R-free | 0.26920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gb8 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.741 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8_1056)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.590 | 4.740 |
High resolution limit [Å] | 4.500 | 4.500 |
Rmerge | 0.150 | 0.600 |
Number of reflections | 12112 | |
<I/σ(I)> | 7.1 | 2.4 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | BY MIXING EQUAL VOLUMES OF PROTEIN (8-10 MG/ML) AND RESERVOIR SOLUTION 0.5 M DISODIUM TARTRATE, 50 MM TRIS PH 8.0 |