4BSH
H7N3 Avian Influenza Virus Haemagglutinin in Complex with Human Receptor Analogue 6'-SLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 115.897, 115.897, 297.497 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 59.830 - 2.250 |
| R-factor | 0.23039 |
| Rwork | 0.229 |
| R-free | 0.25757 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ti8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.075 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.830 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.090 | 0.590 |
| Number of reflections | 36391 | |
| <I/σ(I)> | 13.6 | 2.23 |
| Completeness [%] | 98.6 | 97.9 |
| Redundancy | 6.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 291 | 291 K, 0.1 M PIPES PH 7.0, 2.2 M AMMONIUM SULFATE, 1% PEG 400 |
