4BSF
Human H7N9 Influenza Virus Haemagglutinin in Complex with Avian Receptor Analogue 3'-SLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 116.160, 116.160, 294.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.880 - 2.760 |
| R-factor | 0.2259 |
| Rwork | 0.223 |
| R-free | 0.27640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ti8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.152 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.550 | 2.910 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Rmerge | 0.100 | 0.610 |
| Number of reflections | 19900 | |
| <I/σ(I)> | 10.77 | 2 |
| Completeness [%] | 99.0 | 99.7 |
| Redundancy | 4.6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 291 | 291 K, 0.1 M PIPES PH 7.0, 2.2 M AMMONIUM SULFATE, 1% PEG 400 |
