4BSA
Crystal Structure of the Haemagglutinin (with Asn-133 Glycosylation) from an H7N9 Influenza Virus Isolated from Humans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | H 3 2 |
Unit cell lengths | 116.130, 116.130, 295.510 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 58.130 - 2.300 |
R-factor | 0.25183 |
Rwork | 0.250 |
R-free | 0.29557 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ti8 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.037 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.130 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.080 | 0.690 |
Number of reflections | 34364 | |
<I/σ(I)> | 10.92 | 2.28 |
Completeness [%] | 99.6 | 99.5 |
Redundancy | 5.7 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 291 | 291 K, 0.1 M PIPES PH 7.0, 2.2 M AMMONIUM SULFATE, 1% PEG 400 |