4BRW
Crystal structure of the yeast Dhh1-Pat1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 105.635, 105.635, 122.068 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.474 - 2.795 |
| R-factor | 0.2063 |
| Rwork | 0.204 |
| R-free | 0.24710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s2m |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.743 |
| Data reduction software | XDS |
| Data scaling software | XDS (CORRECT) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.880 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.080 | 0.760 |
| Number of reflections | 17668 | |
| <I/σ(I)> | 18.3 | 2.8 |
| Completeness [%] | 99.6 | 97.1 |
| Redundancy | 8.6 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 50 MM TRIS PH 8.0, 4% MPD, 200 MM NACL, 25% PEG 400 |
