4BRS

Structure of wild type PhaZ7 PHB depolymerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-09
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	41.400, 199.700, 44.200
Unit cell angles	90.00, 114.20, 90.00

Refinement procedure

Resolution	21.923 - 1.600
R-factor	0.1336
Rwork	0.132
R-free	0.17040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vtv
RMSD bond length	0.012
RMSD bond angle	1.282
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.920	1.640
High resolution limit [Å]	1.600	1.600
Rmerge	0.020	0.120
Number of reflections	81064
<I/σ(I)>	27.39	6.63
Completeness [%]	94.3	81.2
Redundancy	2.5	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5		0.1 M NAOAC (PH 5.0), 0.2 M MGCL2 AND 18 % W/V PEG 6000