4BRR

Crystal structure of the integral membrane diacylglycerol kinase - delta 7.79

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-06
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	75.060, 91.500, 143.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.533 - 2.440
R-factor	0.2195
Rwork	0.218
R-free	0.25220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ze3
RMSD bond length	0.007
RMSD bond angle	0.688
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.060	2.500
High resolution limit [Å]	2.440	2.440
Rmerge	0.080	1.030
Number of reflections	37160
<I/σ(I)>	8.6	2.1
Completeness [%]	99.0	99.4
Redundancy	3.3	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.6	277	4-6 %(V/V) 2-METHYL-2, 4-PENTANEDIOL (MPD), 0.1 M SODIUM CHLORIDE, 60 MM MAGNESIUM ACETATE, 0.1 M SODIUM CITRATE/HCL PH 5.6. CRYSTALLIZED USING THE IN MESO (LIPIDIC CUBIC PHASE) METHOD AT 4 DEGREES CELCIUS WITH THE 7.9 MONOACYLGLYCEROL (7.9 MAG) AS THE HOSTING LIPID.