4BR7
Legionella pneumophila NTPDase1 crystal form I, open, AMPNP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 102.853, 102.853, 74.779 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.610 - 1.800 |
R-factor | 0.16377 |
Rwork | 0.163 |
R-free | 0.20941 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.021 |
RMSD bond angle | 1.978 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.000 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.060 |
Number of reflections | 42613 |
<I/σ(I)> | 12.6 |
Completeness [%] | 100.0 |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.7 | 30% PEP 629 (PENTAERYTHRITOL PROPOXYLATE 17/8 PO/OH), 100MM NAHEPES PH 7.7, 50MM MGCL2 |