4BR1
Protease-induced heterodimer of human triosephosphate isomerase.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-11-09 |
| Detector | MARRESEARCH MX-300 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.301, 77.317, 87.311 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.658 - 1.900 |
| R-factor | 0.1786 |
| Rwork | 0.177 |
| R-free | 0.21740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jk2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.800 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.280 |
| Number of reflections | 37152 | |
| <I/σ(I)> | 15.2 | 5.9 |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 7.9 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 100 MM TRIS PH 8.5, 30% POLYETHYLENE GLYCOL 4000, 200 MM SODIUM ACETATE TRIHYDRATE |
