4BQQ
Protein crystal structure of the N-terminal and recombinase domains of the Streptomyces temperate phage serine recombinase, fC31 integrase.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-24 |
Detector | ADSC CCD |
Spacegroup name | P 43 |
Unit cell lengths | 96.040, 96.040, 117.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 96.040 - 2.150 |
R-factor | 0.21963 |
Rwork | 0.218 |
R-free | 0.24427 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.010 |
RMSD bond angle | 1.159 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | SHELX |
Refinement software | REFMAC (5.6.0111) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 96.040 | 2.210 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.050 | 0.650 |
Number of reflections | 56465 | |
<I/σ(I)> | 25.1 | 2.6 |
Completeness [%] | 98.3 | 97.3 |
Redundancy | 4 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 0.1M SODIUM CITRATE PH5.5, 1.55M DI AMMONIUM HYDROGEN PHOSPHATE |