4BQO
Structural insights into WcbI, a novel polysaccharide biosynthesis enzyme. Native protein without disulfide bond between COA and Cys14.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-23 |
Detector | ADSC CCD |
Spacegroup name | P 1 |
Unit cell lengths | 47.200, 67.900, 71.900 |
Unit cell angles | 62.90, 76.20, 69.80 |
Refinement procedure
Resolution | 44.000 - 1.560 |
R-factor | 0.17894 |
Rwork | 0.177 |
R-free | 0.21290 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.011 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.700 | 1.600 |
High resolution limit [Å] | 1.560 | 1.560 |
Rmerge | 0.050 | 0.570 |
Number of reflections | 92966 | |
<I/σ(I)> | 13 | 2.2 |
Completeness [%] | 92.0 | 72.6 |
Redundancy | 3.9 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 10% (W/V) PEG 8000, 75 MM LICL, 75 MM MGCL2, 0.05 M HEPES PH 7.0-8.0. |