4BQO
Structural insights into WcbI, a novel polysaccharide biosynthesis enzyme. Native protein without disulfide bond between COA and Cys14.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-23 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.200, 67.900, 71.900 |
| Unit cell angles | 62.90, 76.20, 69.80 |
Refinement procedure
| Resolution | 44.000 - 1.560 |
| R-factor | 0.17894 |
| Rwork | 0.177 |
| R-free | 0.21290 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.700 | 1.600 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.050 | 0.570 |
| Number of reflections | 92966 | |
| <I/σ(I)> | 13 | 2.2 |
| Completeness [%] | 92.0 | 72.6 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 10% (W/V) PEG 8000, 75 MM LICL, 75 MM MGCL2, 0.05 M HEPES PH 7.0-8.0. |
